Search results for "flight mass spectrometry"

showing 10 items of 31 documents

Improvement of a rapid direct blood culture microbial identification protocol using MALDI-TOF MS and performance comparison with SepsiTyper kit

2018

Fast diagnosis of pathogens is critical to guarantee the most adequate therapy for infections; bacterial culture methods, which constitute the actual gold standard, are precise and sensitive but rather slow. Today, new methods have been made available to enable faster diagnosis, with the Matrix-Assisted Laser Desorption Ionization-Time Of Flight Mass Spectrometry (MALDI-TOF MS) technique being the most promising. Even if simpler and faster than traditional bacterial culture methods, analysis of positive blood cultures via MALDI-TOF MS requires a preliminary extraction process of samples. In this study, we compared two extraction protocols for bacterial identification directly from positive …

0301 basic medicineMicrobiology (medical)Time FactorsComputer science030106 microbiologyBacteremiaClinical diagnostic laboratorySensitivity and SpecificityMicrobiology03 medical and health sciencesSpecies SpecificitymedicineHumansBlood cultureOverall performanceMolecular BiologyProtocol (science)Bacteriological TechniquesChromatographyBacteriamedicine.diagnostic_testDiagnostic Tests RoutineGold standard (test)Matrix-assisted laser desorption/ionizationIdentification (information)Blood CulturePathogens identificationSpectrometry Mass Matrix-Assisted Laser Desorption-IonizationPerformance comparisonCosts and Cost AnalysisGenus and species identificationMatrix- assisted laser desorption ionization time of flight mass spectrometryJournal of Microbiological Methods
researchProduct

Direct identification of microorganisms from thioglycolate broth by MALDI-TOF MS.

2017

We developed an easy MALDI-TOF MS-based assay to identify microorganisms directly from thioglycolate broth. A total of 101 positive thioglycolate broths inoculated with 15 different kinds of samples were evaluated. In 91 samples (90.1%), direct MALDI-TOF MS identifications were the same as those obtained after conventional laboratory procedures including subcultures. In 10 samples misidentified by direct processing, yeasts or mixed cultures grew in the thioglycolate subcultures, or high cellular debris hampered a correct analysis. This rapid method can provide a fast, clinically- relevant species-level identification without disturbing the daily workflow in clinical microbiology laboratorie…

0301 basic medicinePhysiologyMicroorganismlcsh:MedicinePathology and Laboratory MedicineNervous SystemMass SpectrometryAnalytical Chemistrychemistry.chemical_compoundSpectrum Analysis TechniquesMicrobial PhysiologyCandida albicansMedicine and Health SciencesMatrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometrylcsh:ScienceCerebrospinal FluidMultidisciplinaryChemistryMicrobial Growth and DevelopmentClinical Laboratory SciencesBody FluidsClinical microbiologyChemistryClinical LaboratoriesThioglycolatesPhysical SciencesAnaerobic bacteriaAnatomyCellular DebrisResearch ArticleClinical PathologyThioglycolate broth030106 microbiologyAnaerobic BacteriaResearch and Analysis MethodsMicrobiology03 medical and health sciencesSigns and SymptomsDiagnostic MedicineGram Negative BacteriaLaboratory methodsChromatographyBacteriaBacterial Growthlcsh:ROrganismsBiology and Life SciencesBacteriologyCulture MediaMatrix-assisted laser desorption/ionizationAbscessesClinical MicrobiologySpectrometry Mass Matrix-Assisted Laser Desorption-Ionizationlcsh:QLaboratoriesDevelopmental BiologyPLoS ONE
researchProduct

Metabolic signatures across the full spectrum of non-alcoholic fatty liver disease.

2022

Funder: European Commission

ALTtype 2 diabetes mellitusROC receiving operator characteristicaspartate aminotransferaseHSDLDL low-density lipoproteinUHPLC ultrahigh-performance liquid chromatographyROCHCCNon-alcoholic steatohepatitisGCPCANASHGastroenterology2-HB 2-hydroxybutanoic acid; 3-HB 3-hydroxybutanoic acid; ALT alanine aminotransferase; AST aspartate aminotransferase; CE cholesterol ester; Cer ceramide; FFA free fatty acid; FLIP Fatty Liver Inhibition of Progression; Fibrosis; GC gas chromatography; HCC hepatocellular carcinoma; HSD honest significant difference; LC lipid cluster; LDL low-density lipoprotein; LM lipid and metabolite; LMC lipid metabolite and clinical variable; LPC lysophosphatidylcholine; Lipidomics; Mass spectrometry; Metabolomics; NAFL non-alcoholic fatty liver; NAFLD non-alcoholic fatty liver disease; NAS NASH activity score; NASH non-alcoholic steatohepatitis; NIDDK NASH-CRN National Institute of Digestive Diseases and Kidney NASH Clinical Research Network; NRR non-rejection rate; Non-alcoholic steatohepatitis; PC(O) ether PC; PC phosphatidylcholine; PCA principal component analysis; PE phosphatidylethanolamine; QTOFMS quadrupole-time-of-flight mass spectrometry; ROC receiving operator characteristic; SAF steatosis activity and fibrosis; SM sphingomyelin; T2DM type 2 diabetes mellitus; TG triacylglycerol; UHPLC ultrahigh-performance liquid chromatographySAFSAF steatosis activity and fibrosisLM lipid and metabolitehonest significant differenceHSD honest significant differenceTG triacylglycerolnon-rejection ratecholesterol esterPCPEGC gas chromatographyfree fatty acidFLIPNASH non-alcoholic steatohepatitisNIDDK NASH-CRN National Institute of Digestive Diseases and Kidney NASH Clinical Research NetworkBIOMARKERST2DMPE phosphatidylethanolamineLDLlipidNAFLDFFA free fatty acid2-HBMetabolomicsNAFL non-alcoholic fatty liverLMCphosphatidylcholineScience & TechnologySM sphingomyelinGastroenterology & HepatologyMass spectrometryactivitynutritional and metabolic diseasesT2DM type 2 diabetes mellitusACIDSreceiving operator characteristicdigestive system diseasesquadrupole-time-of-flight mass spectrometryLC lipid clusterlow-density lipoproteinNAS2-HB 2-hydroxybutanoic acidNAS NASH activity scoreQTOFMSether PCNRRSCORING SYSTEMprincipal component analysisgas chromatographyLC2-hydroxybutanoic acidPROGRESSIONAST aspartate aminotransferaseLMPC phosphatidylcholinePC(O)MARKERSUHPLCsteatosisTOOLImmunology and AllergyINSULIN-RESISTANCECerSMFatty Liver Inhibition of Progressionhepatocellular carcinoma2-HB 2-hydroxybutanoic acid NIDDK NASH-CRN National Institute of Digestive Diseases and Kidney NASH Clinical Research Network NRR non-rejection rate Non-alcoholic steatohepatitis PC(O) ether PC PC phosphatidylcholine PCA principal component analysis PE phosphatidylethanolamine QTOFMS quadrupole-time-of-flight mass spectrometry ROC receiving operator characteristic SAF steatosis activity and fibrosis SM T2DM type 2 diabetes mellitus TG triacylglycerol UHPLC ultrahigh-performance liquid chromatographyultrahigh-performance liquid chromatographyCELPC3-HBNAFLnon-alcoholic fatty liverTGtriacylglycerolNRR non-rejection rateLife Sciences & BiomedicineNAFLD non-alcoholic fatty liver diseaseFLIP Fatty Liver Inhibition of Progressionalanine aminotransferasemetaboliteCer ceramideCE cholesterol estersphingomyelinlysophosphatidylcholineand fibrosisALT alanine aminotransferaseInternal MedicineceramideNational Institute of Digestive Diseases and Kidney NASH Clinical Research NetworkAST3-HB 3-hydroxybutanoic acidQTOFMS quadrupole-time-of-flight mass spectrometryPCA principal component analysisLPC lysophosphatidylcholineHepatologynon-alcoholic fatty liver diseaseand clinical variablePC(O) ether PC3-hydroxybutanoic acidFibrosisNASH activity scoreNIDDK NASH-CRNlipid clusterlipid and metabolitephosphatidylethanolamineLipidomicsLMC lipid metabolite and clinical variableFFAHCC hepatocellular carcinomaJHEP reports : innovation in hepatology
researchProduct

Rapid antibiotic susceptibility testing on blood cultures using MALDI-TOF MS

2018

International audience; Antibiotic resistance is a major public health problem requiring the early optimization of antibiotic prescriptions. Matrix-Assisted Laser Desorption Ionization-Time Of Flight Mass Spectrometry (MALDI-TOF MS) has been shown to accurately identify bacteria from positive blood culture. Here, we developed a rapid detection of Escherichia coli resistance to amoxicillin (AMX) and cefotaxime (CTX) from positive blood culture based on MALDI-TOF MS. Potential sparing of broad-spectrum antibiotics was also evaluated. We tested 103 E. coli-positive blood cultures. Blood cultures were subculture 1-hour in antibiotic-free rich liquid media before further incubation with and with…

Agar Dilution Method0301 basic medicineTime FactorsCefotaximePhysiologyAntibioticslcsh:MedicineCefotaximeDrug resistancemedicine.disease_causeMass SpectrometryAnalytical ChemistrySpectrum Analysis TechniquesAntibioticsMicrobial PhysiologyMedicine and Health SciencesBlood cultureMatrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometrylcsh:ScienceMultidisciplinarymedicine.diagnostic_testbiologyAntimicrobialsChemistryMicrobial Growth and DevelopmentDrugsMatrix-Assisted Laser Desorption Ionization Mass SpectrometryBody FluidsAnti-Bacterial Agents3. Good healthChemistryBlood[SDV.MP]Life Sciences [q-bio]/Microbiology and ParasitologyPhysical SciencesAnatomyResearch Articlemedicine.drugmedicine.drug_class030106 microbiologyMicrobial Sensitivity TestsResearch and Analysis MethodsMicrobiologyAntibiotic Susceptibility TestingMicrobiology03 medical and health sciencesMicrobial ControlDrug Resistance BacterialEscherichia colimedicineHumansEscherichia coliPharmacologyBacteriological TechniquesBacterial Growthlcsh:RBiology and Life SciencesAmoxicillinAmoxicillinbiology.organism_classificationPharmacologic AnalysisBlood CultureAntibiotic ResistanceSpectrometry Mass Matrix-Assisted Laser Desorption-Ionizationlcsh:QAntimicrobial ResistanceSubculture (biology)BacteriaDevelopmental BiologyPLOS ONE
researchProduct

Validation of a novel automatic deposition of bacteria and yeasts on MALDI target for MALDI-TOF MS-based identification using MALDI Colonyst robot

2017

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) -based identification of bacteria and fungi significantly changed the diagnostic process in clinical microbiology. We describe here a novel technique for bacterial and yeast deposition on MALDI target using an automated workflow resulting in an increase of the microbes' score of MALDI identification. We also provide a comparison of four different sample preparation methods. In the first step of the study, 100 Gram-negative bacteria, 100 Gram-positive bacteria, 20 anaerobic bacteria and 20 yeasts were spotted on the MALDI target using manual deposition, semi-extraction, wet deposition onto 70% formic …

Atmospheric ScienceCarboxylic Acidslcsh:MedicineAnaerobic BacteriaResearch and Analysis MethodsMicrobiologyMass SpectrometryAnalytical ChemistryAutomationSpectrum Analysis TechniquesYeastsEnvironmental Chemistrylcsh:ScienceMatrix-Assisted Laser Desorption Ionization Time-of-Flight Mass SpectrometryGram Negative BacteriaBacteriaOrganic Compoundslcsh:REcology and Environmental SciencesGram Positive BacteriaFormic AcidOrganic ChemistryOrganismsFungiChemical CompoundsBiology and Life SciencesEukaryotaBacteriologyRoboticsYeastChemistryAtmospheric ChemistrySpectrometry Mass Matrix-Assisted Laser Desorption-IonizationPhysical SciencesEarth Scienceslcsh:QAcid DepositionAcidsResearch ArticlePLoS ONE
researchProduct

Rapid and Sensitive Identification of Major Histocompatibility Complex Class I-associated Tumor Peptides by Nano-LC MALDI MS/MS

2005

Identification of major histocompatibility complex (MHC)-associated peptides recognized by T-lymphocytes is a crucial prerequisite for the detection and manipulation of specific immune responses in cancer, viral infections, and autoimmune diseases. Unfortunately immunogenic peptides are less abundant species present in highly complex mixtures of MHC-extracted material. Most peptide identification strategies use microcapillary LC coupled to nano-ESI MS/MS in a challenging on-line approach. Alternatively MALDI PSD analysis has been applied for this purpose. We report here on the first off-line combination of nanoscale (nano) LC and MALDI TOF/TOF MS/MS for the identification of naturally proce…

CellsCellPeptideHuman leukocyte antigenMajor histocompatibility complexSensitivity and SpecificityBiochemistryEpitopeAnalytical ChemistryMajor Histocompatibility ComplexImmune systemCell Line TumormedicineHumansAmino Acid SequenceCarcinoma Renal CellMolecular Biologychemistry.chemical_classificationbiologyHistocompatibility Antigens Class IFlow CytometryMolecular biologyKidney NeoplasmsPeptide FragmentsNeoplasm Proteinsmedicine.anatomical_structurechemistrySpectrometry Mass Matrix-Assisted Laser Desorption-Ionizationbiology.proteinTime-of-flight mass spectrometryAntibodyMolecular & Cellular Proteomics
researchProduct

Properties and performance of a quadrupole mass filter used for resonance ionization mass spectrometry

1998

Abstract The performance of commercial quadrupole mass spectrometers (QMS) with a number of imperfections, as compared to the ideal hyperbolic geometry, has been characterized using the computer simulation program simion 3d version 6.0. The analysis of simulated QMS geometries focuses primarily on modeling of the internal potential, the study of field deviations, and the influence of finite length on performance of the QMS. The computer simulation of ion trajectories in the QMS field yields predictions for optimum working conditions and provides estimates for the resolving power and the maximum isotopic abundance sensitivity. Experimental measurements that confirm these expectations are pre…

ChemistrySelected reaction monitoringAnalytical chemistryThermal ionization mass spectrometryCondensed Matter PhysicsMass spectrometryTriple quadrupole mass spectrometerComputational physicsSelected ion monitoringPhysical and Theoretical ChemistryTime-of-flight mass spectrometryInstrumentationQuadrupole mass analyzerSpectroscopyHybrid mass spectrometerInternational Journal of Mass Spectrometry
researchProduct

Measurement of nanoparticle mass distributions by laser desorption/ionization time-of-flight mass spectrometry.

2011

In this paper, access to the mass distribution analysis of nanoparticles is described based on laser desorption/ionization and time of flight mass spectrometry. Two examples are given, demonstrating the accurate mass distribution analysis of nanoparticles fabricated both ex situ and in situ during the laser-assisted desorption process. The potentials and the limitations of the method are discussed, with special emphasis on carbonaceous clusters and molecules.

Desorption electrospray ionizationStatic secondary-ion mass spectrometryMatrix-assisted laser desorption electrospray ionizationChemistryAnalytical chemistryGeneral Materials ScienceTime-of-flight mass spectrometryCondensed Matter PhysicsMass spectrometrySoft laser desorptionIon sourceAmbient ionizationJournal of physics. Condensed matter : an Institute of Physics journal
researchProduct

Environmental and food applications of LC-tandem mass spectrometry in pesticide-residue analysis: An overview

2003

An overview is given on pesticide-residue determination in environmental and food samples by liquid chromatography/mass spectrometry/mass spectrometry (LC/MS/MS). Pesticides comprise a large number of substances that belong to many completely different chemical groups, the only common characteristic is that they are effective against pests. They still constitute a challenge in MS because there is no collective pathway for fragmentation. A brief introduction to the theory of tandem MS permits a discussion of which parameters influence the ionization efficiency when the ions are subjected to different actions. Emphasis is placed on the different tandem MS instruments: triple and ion-trap quad…

Detection limitChromatographyPesticide residueTandemChemistryCondensed Matter PhysicsTandem mass spectrometryMass spectrometryGeneral Biochemistry Genetics and Molecular BiologyAnalytical ChemistrySample preparationQuadrupole ion trapTime-of-flight mass spectrometrySpectroscopyMass Spectrometry Reviews
researchProduct

Neutron cross section measurements at n_TOF for ADS related estudies

2005

A neutron Time-of-Flight facility (n_TOF) is available at CERN since 2001. The innovative features of the neutron beam, in particular the high instantaneous flux, the wide energy range, the high resolution and the low background, make this facility unique for measurements of neutron induced reactions relevant to the field of Emerging Nuclear Technologies, as well as to Nuclear Astrophysics and Fundamental Nuclear Physics. The scientific motivations that have led to the construction of this new facility are here presented. The main characteristics of the n_TOF neutron beam are described, together with the features of the experimental apparata used for cross-section measurements. The main res…

HistoryAstrofísica nuclearNeutron cross sectionsNuclear transmutationNuclear engineeringNuclear TheoryNuclear physicsNeutrons -- SeccionsEducationNuclear physicsRadiació ionitzant -- Mesures de seguretatRadioactive wastesNeutron cross sectionNuclear astrophysicsNeutronNuclear ExperimentPhysicsLarge Hadron Collider:Física [Àrees temàtiques de la UPC]Time-of-flight mass spectrometryRadioactive wasteNeutron radiationWaste disposalResidus radioactiusComputer Science ApplicationsShielding (Radiation)Physics::Accelerator PhysicsNuclear astrophysicsFísica nuclearNucleon
researchProduct